SEMPRE RB
PROGRAM
Sempre RB combines latest biotechnology innovation in cells, subcellular therapy, and genetic modulation.
Main elements of Sempre RB program are autologous, which ensures safety. Tissue regeneration, cryopreservation and subsequent retransplantation provide rejuvenation and life-long effects.
Normal cells maintain the controlled balance within cell metabolism because the optimal building of cell components and maintenance of cellular energy levels are essential for cell activity. Aging is accompanied by a general decline of cellular functionality and disruption of cellular homeostasis.
Sempre X and Y treatments prevent both cellular ageing and reducing its consequences by restoring homeostasis within aged tissues. The Sempre RB with stem cells and growth factors should modulate the complex interplay between cells in the tissues and improve tissue-level metabolic dysfunction associated with aging and thus prolong healthy life in humans.
The initial, most important part of the procedure, phase 1 to phase 4, is done at the beginning of the program. The yearly autologous support programs for SEMPRE X involves using PLRP (Platelet-Leukocyte Rich Plasma) treatment and/or Mesenchymal Stem Cells (MSC) to treat and rejuvenate peripheral tissues such as skin and hair but also the Platelet Rich Plasma vaginal therapy in women (Orgasm shot, O-shot).
The initial, most important part of the procedure, phase 1 to phase 4, is done at the beginning of the program. The yearly autologous support programs for SEMPRE Y involves using PLRP (Platelet-Leukocyte Rich Plasma) treatment and/or Mesenchymal Stem Cells (MSC) to treat and rejuvenate peripheral tissues such as skin and hair but also the Platelet Rich Plasma penile injection therapy in men (P-Shot).
Sempre RB consists of 6 phases: obtaining of the growth factors and gonadal tissue; in vitro activation of the obtained tissue; obtaining of the bone marrow with mesenchymal stem cells and ultrasound-guided orthotropic retransplantation of the gonadal tissue and stem cells; slow freezing of the stem cells and gonadal tissue, quality control of the stored material; follow up and re-boosting; subsequent retransplantation of the stored tissues and local/systemic application of stem cells and exosomes.
Day 1 for the participant:
The patient is received at the clinic, and laparoscopic ovarian cortical resection (female) / ambulatory testicle biopsy (male) under anesthesia is performed.
The aim is to obtain a small piece of the reproductive/endocrine tissue that will be further treated with autologous sources of treatment-using patients’ own stem cells and growth factors to obtain rejuvenation effects.
The procedure is performed with minimal surgical biopsy.
During the surgery, a sample of blood from the cubital vein is taken and used to obtain growth factors from the patient’s own blood (called platelet-rich plasma) by a special separation method that will be used to treat gonadal
tissue (for tissue activation and retransplantation).
Day 2 for the participant:
The patient rests while gonadal tissue is processed for activation in the laboratory. Growth factors that were prepared the day before are used now for tissue activation.
Day 3 for the participant:
Another small surgical procedure is performed to obtain bone marrow samples with mesenchymal stem cells from tibia or iliac crest bone under anesthesia. The bone marrow samples are further processed for retransplantation as
well as microvesicles and exosomes, which are derived from mesenchymal stem cells.
At the same time, in the laboratory, gonadal tissue is processed for retransplantation. One part of the bone marrow tissue is used together with processed gonadal tissue for orthotropic retransplantation in the ovaries/testicles
by the ultrasound-guided procedure.
That is the last part of the third day of the clinical program for the patient.
After discharge from the clinic, a patient receives an individually designed nutrition and workout regimen for energy mitochondrial boosting.
In the laboratory, specifically processed gonadal tissue together with mesenchymal stem cells and exosomes are frozen by a slow freezing process and sent to our tissue bank under special conditions where it remains until further use.
In the tissue bank, the quality of cells is checked first, and then the tissue is vitrified (frozen under special conditions).
During the years of storage, the bank continuously monitors the quality of storage conditions.
Follow up and re-boosting program begins.
Periodic testing is done to monitor a variety of parameters expected to give insight into relevant body functions. These tests can be completed at specified intervals at a laboratory which is most suitable for the program participant.
An optional annual autologous support program offers boosting exosome therapies, the use of PLRP (platelet-leukocyte rich plasma) treatment, and/or mesenchymal stem cells (MSC) to treat and rejuvenate peripheral tissues such
as skin, hair, but also the platelet-rich plasma injection therapy in men and women, etc.
In some cases, plasmapheresis and partial blood exchange is performed in order to improve outcome.
Implemented several years after the first part of the program - in situations when the aging process begins (based on laboratory monitoring of the function and genetics of the organism). It consists of autologous, gonadal tissue retransplantation, as well as local/systemic application of stem cells and exosomes. Stem cells can be multiplicated before transplantation.